Involvement of Gicerin, a Cell Adhesion Molecule, in the Hematogenous Metastatic Activities of a Melanoma Cell Line
Naoki Kirimura, Yuka Kubota, Hatsuki Shiba, Kazuhide Adachi, Yasuhiro Tsukamoto

Gicerin is an immunoglobulin superfamily cell adhesion molecule that plays a role in development via its cell adhesive activities. After maturation, the gicerin expressionalmost disappears in most organs, except for the muscle and endothelial cells. Interestingly, various neoplastic cells strongly express gicerin in their cell membranes, indicating a potential function of gicerin in the development of malignancy. In the present study, we analyzed the potential role of gicerin in the metastasis of melanoma. Gicerin was found to be expressed in the cell membrane of the B16 implantable melanoma cell line. In addition, cell adhesion activity of B16was clearly promoted on gicerin proteins and HUVEC cells, an endothelial cell line. B16 cells were then implanted intravenously into nude mice in order to evaluate the metastatic activity of gicerin in vivo. Following implantation, metastatic lesions were frequently observed in the pulmonary tissues, whereas tumor emboli and extravasation were found in the pulmonary blood vessels. In contrast, no metastatic lesions were detected in the pulmonary tissues of the mice injected with B16 cells preincubated with anti-gicerin polyclonal antibodies. These findings suggest that gicerin enhances the pulmonary metastatic properties of melanoma cells by promoting endothelium-tumor interactions.

Full Text: PDF     DOI: 10.15640/aijb.v2n3-4a4