Early Developmental Stages of Homalothecium sericeum (Hew.) Schimp (Brachytheciaceae) under in vitro Conditions
Bengi Erdağ, Münire Nihan Bağdatli, İlknur Kuzu, Yelda Emek

In this study, spore germination features, protonemal and gametophytic development stages of Homalothecium sericeum (Hew.) Schimp. had been investigated. Effects of plant growth regulators on colony size and number of gametophyte per colony were also studied. Spore germination is in exosporic type and dependent upon on light. The highest germination percentage is obtained in Distilled water exposed to 24 ± 2 °C and 16:8 photoperiod conditions. It is followed by 1/8 MS, ¼ MS, ½ MS and full strength MS media. Inspite of observing the highest germination percentage in Distilled water, this was not used in the further experiments because of weak chloronemal development and no colonization has been observed. Among the MS media in four different strength, the best colonial growth was obtained in full strength MS medium. In terms of the level of colony growth, the MS medium was followed by the ½ MS medium. The ¼ and 1/8 MS media inhibited the forward growth of protonemata. Sporelling type of H. sericeum is Bryum type due to spore germination and protonemal form with long cylindiric cells. Response to cytokinins in colonial growth and gametophyte differentiation was observed as abnormal bud formation. Auxine type and concentration effected colony size and number of gametophyte per colony. At the and of sixth months, maximum colony size and maximum number of gametophyte per colony has been held in MS medium containing 1mg/L IAA.

Full Text: PDF     DOI: 10.15640/aijb.v3n1a1