Effects of Hypothermic Temperatures on Tau Hyperphosphorylation as a Model of Alzheimer’s Disease in SH-SY5Y Cells
Samantha Johnson; Zin M. Htway, PhD; Hector E. Hernandez, PhD

Abstract
Alzheimer’s disease, among other neurodegenerative diseases, is associated with tau hyperphosphorylation. This study evaluated SH-SY5Y cells grown in hypothermic temperatures (30 ºC) and compared them against cells in normothermic conditions (37 ºC) to assess tau hyperphosphorylation at varying temperatures in a time-course analysis. Enzyme-linked immunosorbent assay (ELISA) was used to measure the expression of the pT181 isoform, a biomarker of tau hyperphosphorylation. This study found that hypothermic incubation does increase tau hyperphosphorylation best at 18 hours of incubation. These results suggest that hypothermic conditions can serve as an in-vitro model for Alzheimer’s research.

Full Text: PDF DOI: 10.15640/aijb.v6n1a4